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ph10 tris buffer  (Millipore)


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    Structured Review

    Millipore ph10 tris buffer
    Ph10 Tris Buffer, supplied by Millipore, used in various techniques. Bioz Stars score: 96/100, based on 532 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ph10+tris+buffer/pH+10+Buffer/pmc08062362-94-7-10
    Average 96 stars, based on 532 article reviews
    ph10 tris buffer - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Staining:

    Article Title: Stroma-regulated HMGA2 is an independent prognostic marker in PDAC and AAC
    Article Snippet: IHC was performed on a Ventana autostainer (Ventana Benchmark Discovery, NexES V10.6; Tuscon, AZ, USA) using the rabbit monoclonal anti-HMGA2 antibody (clone D1A7, #8179 Cell Signaling, Danvers, MA, USA; 1 : 400 dilution) or the rabbit monoclonal anti-PDGFR β antibody (clone 28E1, #3169 Cell Signaling; 1 : 100 dilution) diluted in Antibody Diluent Buffer (Antibody diluent, Ventana). .. The staining protocol included an extended antigen retrieval step with CC1/pH9 buffer (Discovery CC1, Ventana) for HMGA2, or with pH10 Tris buffer (Sigma-Aldrich and Merck Kgaa, Darmstadt, Germany) for PDGFR β staining, incubation with the primary antibody for 1 h at room temperature (RT) and incubation with the secondary antibody (OmniMab anti-Rb-HRP, Ventana) for 32 min at RT. ..

    Article Title: Fibroblasts in urothelial bladder cancer define stroma phenotypes that are associated with clinical outcome
    Article Snippet: The rabbit monoclonal anti-PDGFRb antibody (clone 28E1, #3169 Cell Signaling, Danvers MA, US; 1:100 dilution), the rabbit monoclonal anti-PDGFRa antibody (clone D13C6, #5241 Cell Signaling, Danvers MA, US; 1:100 dilution) and the monoclonal rabbit anti-CD90 antibody (clone EPR3132, #ab92574, Abcam, Cambridge, UK) were diluted in Antibody Diluent Buffer (Antibody diluent, Ventana, Tuscon, Arizona, US). .. An extended antigen retrieval step with Cell Conditioning Buffer 1 (CC1) (Ventana) was included for the PDGFRa and CD90 staining and with pH10 Tris buffer (Sigma-Aldrich and Merck Kgaa, Darmstadt, Germany) for the PDGFRb staining. .. The staining protocol was performed with the Discovery OmniMap anti-rabbit HRP (RUO) detection kit (Ventana), including incubation with the primary antibody for 1 h and incubation with the secondary anti-rabbit HRP antibody for 32 min. Counterstaining was done with Hematoxylin II for 10 min and subsequent bluing for 6 min (Ventana).

    Incubation:

    Article Title: Stroma-regulated HMGA2 is an independent prognostic marker in PDAC and AAC
    Article Snippet: IHC was performed on a Ventana autostainer (Ventana Benchmark Discovery, NexES V10.6; Tuscon, AZ, USA) using the rabbit monoclonal anti-HMGA2 antibody (clone D1A7, #8179 Cell Signaling, Danvers, MA, USA; 1 : 400 dilution) or the rabbit monoclonal anti-PDGFR β antibody (clone 28E1, #3169 Cell Signaling; 1 : 100 dilution) diluted in Antibody Diluent Buffer (Antibody diluent, Ventana). .. The staining protocol included an extended antigen retrieval step with CC1/pH9 buffer (Discovery CC1, Ventana) for HMGA2, or with pH10 Tris buffer (Sigma-Aldrich and Merck Kgaa, Darmstadt, Germany) for PDGFR β staining, incubation with the primary antibody for 1 h at room temperature (RT) and incubation with the secondary antibody (OmniMab anti-Rb-HRP, Ventana) for 32 min at RT. ..

    Article Title: Prognostic and predictive impact of stroma cells defined by PDGFRb expression in early breast cancer: results from the randomized SweBCG91RT trial
    Article Snippet: The Ventana Benchmark Discovery autostainer system (NexES V10.6) was used for immunohistochemical staining of PDGFRb on 4 μm freshly cut sections from formalin-fixed paraffin embedded tissue microarray (TMA) blocks. .. The protocol included extended antigen retrieval with pH10 Tris buffer (Sigma-Aldrich and Merck Kgaa, Darmstadt, Germany) and incubation for 1 h at 37 °C with the primary antibody (rabbit monoclonal anti-PDGFRb antibody, clone 28E1, #3169 Cell Signaling, Danvers MA, US) diluted at 1:100 dilution in Discovery Antibody Diluent (Ventana, Tuscon, Arizona, US). .. Chromogenic detection was performed using the Discovery OmniMap anti-rabbit HRP (RUO) kit (Ventana) with secondary antibody incubation for 32 min at room temperature.



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    ph10 tris buffer  (Millipore)
    96
    Millipore ph10 tris buffer
    Ph10 Tris Buffer, supplied by Millipore, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ph10+tris+buffer/pH+10+Buffer/pmc08062362-94-7-10
    Average 96 stars, based on 1 article reviews
    ph10 tris buffer - by Bioz Stars, 2026-09
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    ph10 tris buffer  (Merck KGaA)
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    Multivariable analysis for 5-year survival data, including stroma-marker defined patient clusters.
    Ph10 Tris Buffer, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ph10+tris+buffer/ph+10+buffer/pmc06959241-187-18-27
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    ph10 tris buffer - by Bioz Stars, 2026-09
    90/100 stars
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    Image Search Results


    Multivariable analysis for 5-year survival data, including stroma-marker defined patient clusters.

    Journal: Scientific Reports

    Article Title: Fibroblasts in urothelial bladder cancer define stroma phenotypes that are associated with clinical outcome

    doi: 10.1038/s41598-019-55013-0

    Figure Lengend Snippet: Multivariable analysis for 5-year survival data, including stroma-marker defined patient clusters.

    Article Snippet: An extended antigen retrieval step with Cell Conditioning Buffer 1 (CC1) (Ventana) was included for the PDGFRa and CD90 staining and with pH10 Tris buffer (Sigma-Aldrich and Merck Kgaa, Darmstadt, Germany) for the PDGFRb staining.

    Techniques: Marker

    Associations between clinicopathological parameters and stroma marker defined patient clusters.

    Journal: Scientific Reports

    Article Title: Fibroblasts in urothelial bladder cancer define stroma phenotypes that are associated with clinical outcome

    doi: 10.1038/s41598-019-55013-0

    Figure Lengend Snippet: Associations between clinicopathological parameters and stroma marker defined patient clusters.

    Article Snippet: An extended antigen retrieval step with Cell Conditioning Buffer 1 (CC1) (Ventana) was included for the PDGFRa and CD90 staining and with pH10 Tris buffer (Sigma-Aldrich and Merck Kgaa, Darmstadt, Germany) for the PDGFRb staining.

    Techniques: Marker

    ( A ) Association between stroma-marker defined patient clusters and CD8a status based on congruency table analysis. P value is calculated by Fisher’s extact test. ( B ) Forest plot indicating overall survival hazard ratios (HR) with 95% confidence interval (CI) for patients with high numbers of CD8a in reference to those patients with low CD8a number within the whole population or stratified by the stroma-marker defined clusters. HR are based on cox proportional hazards regression models and Wald test (*p < 0.05 is highlighted in bold). ( C ) Kaplan–Meier analysis of the relationship between “low” (grey line) and “high” (black line) CD8a frequency and the 5-year overall survival within the CD90-dominant patient cluster. P-values from log-rank test are given to evaluate differences between the population survival curves. Table indicates the number of individuals at risk at the given time points. The unadjusted “crude” HR with 95% CI is based on Cox regression and Wald test. Adjusted HR included gender, age group, grade and stage as additional categorical variables in the multivariable (MV) regression model.

    Journal: Scientific Reports

    Article Title: Fibroblasts in urothelial bladder cancer define stroma phenotypes that are associated with clinical outcome

    doi: 10.1038/s41598-019-55013-0

    Figure Lengend Snippet: ( A ) Association between stroma-marker defined patient clusters and CD8a status based on congruency table analysis. P value is calculated by Fisher’s extact test. ( B ) Forest plot indicating overall survival hazard ratios (HR) with 95% confidence interval (CI) for patients with high numbers of CD8a in reference to those patients with low CD8a number within the whole population or stratified by the stroma-marker defined clusters. HR are based on cox proportional hazards regression models and Wald test (*p < 0.05 is highlighted in bold). ( C ) Kaplan–Meier analysis of the relationship between “low” (grey line) and “high” (black line) CD8a frequency and the 5-year overall survival within the CD90-dominant patient cluster. P-values from log-rank test are given to evaluate differences between the population survival curves. Table indicates the number of individuals at risk at the given time points. The unadjusted “crude” HR with 95% CI is based on Cox regression and Wald test. Adjusted HR included gender, age group, grade and stage as additional categorical variables in the multivariable (MV) regression model.

    Article Snippet: An extended antigen retrieval step with Cell Conditioning Buffer 1 (CC1) (Ventana) was included for the PDGFRa and CD90 staining and with pH10 Tris buffer (Sigma-Aldrich and Merck Kgaa, Darmstadt, Germany) for the PDGFRb staining.

    Techniques: Marker